FlyBase Recombinant Construct Report: P{lwB}

General Information

Symbol

P{lwB}

FlyBase ID

FBtp0000157

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^P\T.A92a

Size

12.486

Expression Data

lacZ reporter

Associated insertion(s)

115 available

Molecular map

Description and Uses

Description

P{lwB} is an enhancer trap construct that contains at its 5' end the relatively weak P\T promoter fused upstream of sequence encoding the lacZ reporter enzyme (the lacZ sequence is fused in-frame to the second exon of the P\T coding sequence).

(Wilson et al., 1989)

The lacZ reporter encoded by the P{lwB} construct is expected to be localized to the nucleus, because it is fused to the N-terminal 128 amino acid residues of P\T (which have been shown to be sufficient for nuclear localization).

(FlyBase, 1992-)

Construct components

Component

Use(s)

Element type

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Marker(s)

w^+mW.hs

Component allele Ecol\lacZ^P\T.A92a

Transgenic product class

Component

Use(s)

Encoded product / tool

Ecol\lacZ

Description

Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo.

(Wilson et al., 1989)

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

12.486

Left end

P5\'

(Wilson et al., 1989)

Right end

P3\'

(Wilson et al., 1989)

Segments

Num

Orientation

Symbol

Reference

(Wilson et al., 1989)

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^P\T.A92a

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epidermis

(Perrimon et al., 1991)

organism | 10-100% egg length

(Perrimon et al., 1991)

• nucleus

(Perrimon et al., 1991)

extended germ band stage

dorsal ectoderm | segmentally repeated

(Bellen et al., 1989)

embryonic stage 9 -- 12

procephalic neurogenic region

(Bellen et al., 1989)

Additional Information

Statement

Reference

Basal pattern of stripes, restricted to epidermal clusters of cells. Expression localized to nucleus.

(Perrimon et al., 1991)

Expression pattern dependent upon position of insertion in genome. Basal pattern observed in approximately 30% of transformants: in extended germ band embryos, weak expression in dorsal epidermis, stronger in alternate segments.

(Bellen et al., 1989)

Marker for

Reflects expression of

P\T

(Bellen et al., 1989)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{lwB}

(Wilson et al., 1989)

descendant(s)

P{5'lwB}