Tag:AsLOV2(BLITz-1) is an engineered light-regulated TEVp protease target site that consists of the LOV2 flavin binding domain (including the carboxy-terminal Jα helix) of the Avena sativa Phototropin 1 (PT1) gene, in which ENLYFQG, the optimum recognition and cleavage site for the TEVp protease, replaces the C-terminal end of the Jα helix. In the dark, the closed conformation of the LOV2 domain results in the protease target site being inaccessible to TEVp. Upon illumination with blue light, the α-helix unfolds, exposing the protease target site sequence and allowing cleavage by TEVp (PMID:28369042).