FlyBase Insertion Report: Dmel\P{GawB}Eip63F-1[55B]

General Information

Symbol

Dmel\P{GawB}Eip63F-1^55B

Species

D. melanogaster

Name

FlyBase ID

FBti0150060

Feature type

transposable_element_insertion_site

Also Known As

55B-GAL4, 55B, 55B GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Eip63F-1, Scer\GAL4

Viability / fertility

viable

Associated allele(s)

Eip63F-1^55B, Scer\GAL4^55B

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3L ( 63F7 )

Sequence Location

3L:3,926,447..3,926,447

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:3,926,447..3,926,447

(Skora and Spradling, 2010)

Orientation

Cytological location (computed by FlyBase)

63F7 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Brand and Perrimon, 1994)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Eip63F-1

(Skora and Spradling, 2010)

Scer\GAL4

(Niepielko et al., 2012, Zappia et al., 2011, Skora and Spradling, 2010, Zhang et al., 2009, Furriols et al., 2007, Carneiro et al., 2006, Goentoro et al., 2006, Morris et al., 2006, Pai et al., 2006, Wahlstrom et al., 2006, Acebes et al., 2004, Meignin et al., 2004, Ghiglione et al., 2002, Halfon et al., 2002, Reich and Shilo, 2002, Grammont and Irvine, 2001, Johnson Hamlet and Perkins, 2001, Kiger and Ho, 2001, Suzanne et al., 2001, Yoshida et al., 2001, Araujo and Bier, 2000, Jordan et al., 2000, Bryant et al., 1999, Mantrova et al., 1999, Reich et al., 1999, Sureau and Ferveur, 1999, Mantrova and Hsu, 1998, Sapir et al., 1998, Brand, 1997.6.30, Ferveur et al., 1997, Nakayama et al., 1997, Queenan et al., 1997, Ferveur and Sureau, 1996, Morimoto et al., 1996, Ferveur et al., 1995, Brand and Perrimon, 1994, Brand et al., 1994)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Eip63F-1^55B

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^55B

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Ferveur et al., 1995)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval salivary gland

(Brand and Perrimon, 1994)

adult stage

adult midgut

(Ferveur et al., 1997)

crop

(Ferveur et al., 1997)

adult stage & oogenesis stage S9

follicle cell

(Brand and Perrimon, 1994)

adult stage oogenesis stage S8 -- S14

follicle cell | anterior

(Queenan et al., 1997)

oogenesis

follicle cell | anterior

(Pai et al., 2006)

centripetal follicle cell

(Furriols et al., 2007)

oogenesis stage S10A

follicle cell

(Goentoro et al., 2006)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

adult head

(Nakayama et al., 1997)

adult thorax

(Nakayama et al., 1997)

Additional Information

Statement

Reference

Drives expression in anterior follicle cells starting at oogenesis stage S8.

(FlyBase Curators, 2017)

ScerGAL4^55B drives expression in anterior follicle cells.

(Pai et al., 2006)

Head, thoracic and abdominal extracts of virgin females were subjected to Western analysis, using β-gal antibodies.

(Nakayama et al., 1997)

During oogenesis, from stage S8, expressed in follicle cells over the anterior portion of the oocyte.

(Queenan et al., 1997)

ScerGAL4 expression occurs in a stripeof follicle cells around the anterior of the oocyte in a stage S9 eggchamber. Embryonic ScerGAL4 expression is limited to the salivary glands.

(Brand and Perrimon, 1994)

Expression in a stripe of follicle cells around the anterior of the oocyte in stage 9 egg chambers.

(Brand and Perrimon, 1994)

Marker for

Reflects expression of

Reporter construct used in assay

Avic\GFP^2x.EGFP.UAS

(Goentoro et al., 2006)

Egfr^1.UAS.GFP

(Goentoro et al., 2006)

kek1^UAS.GFP

(Goentoro et al., 2006)

Cbl^L.UAS

(Pai et al., 2006)

Cbl^S.UAS

(Pai et al., 2006)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

1803

w^*; P{GawB}Eip63F-1^55B

Kyoto

106513

w^*; P{GawB}Eip63F-1^55B

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

55B

(Skora and Spradling, 2010, Skora and Spradling, 2010, Furriols et al., 2007, Morris et al., 2006, Pai et al., 2006, Wahlstrom et al., 2006)

55B GAL4

(Carneiro et al., 2006, Bryant et al., 1999)

55B Gal4

(Jordan et al., 2000)

55B-GAL4

(Niepielko et al., 2012, Zappia et al., 2011, Yoshida et al., 2001, Araujo and Bier, 2000)

55B-Gal4

(Wang et al., 2015, Zhang et al., 2009, Ghiglione et al., 2002, Halfon et al., 2002, Reich and Shilo, 2002, Johnson Hamlet and Perkins, 2001, Reich et al., 1999)

55B-PGal4

(Acebes et al., 2004)

55BGal4

(Grammont and Irvine, 2001)

GAL 4 line 55B

(Meignin et al., 2004)

GAL4 line 5

(Ferveur et al., 1995)

GAL4-55B

(Goentoro et al., 2006)

GAL4-55B2

(Kiger and Ho, 2001)

Gal4-55B

(Goentoro et al., 2006)

P{GAL4}noeF

P{GawB}55B

(FlyBase, 1992-)

P{GawB}Eip63F-1^55B

(Hartman et al., 2015)

P{w^+mW.hs=GawB}55B

(FlyBase, 1992-)

nonoenocyte-expressed Gal4

(Ferveur et al., 1997)

pGawB^55B

(Brand and Perrimon, 1994)

Name Synonyms

Secondary FlyBase IDs

FBti0001259
FBti0002126
FBti0074349

References (42)

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