Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.
A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.
In the adult brain, AvicGFPvkg-CC00791 protein is observed in glia at the brain surface (surface associated glial cell), in the brain cortex (cell body glial cell), and in glial cells in neuropils of the medulla, lobula, and lobula plate, and in the chiasm dividing the different neuropils. AvicGFPvkg-CC00791 reflects a pan-glial expression pattern for the gene vkg.
Protein trap line.