P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w+mC mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Exelixis analyzed the sequence flanking P{XP}d06981 on three different occasions. One sequence read mapped the P{XP}d06981 insertion to Release 3 coordinate 3R:12879395 . A second read mapped P{XP}d06981 to 3R:19043238 . A sequence read from the proximal side of the recombined P{XP-U}Exel6176 insertion associated with Df(3R)Exel6176 mapped it to 3R:12879384 . These results suggest that the 3R:19043238 position is incorrect and represents a sample tracking error of some kind.
Location 3R:19052581-19052582 confirmed by FlyBase alignment of dbGSS accession CZ475462 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.