P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w+mC mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Exelixis, Inc. determined the insertion site of P{XP}d00641 to be at Release 3 genomic coordinate 21509695 on arm 2L.The Gene Disruption project determined the insertion site of P{XP}d00641 to be at Release 3 genomic coordinate 21509890 on arm 2L.
Location 2L:21664362-21664363 confirmed by FlyBase alignment of dbGSS accession CZ471721 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.