FB2024_03 , released June 25, 2024
Insertion: Dmel\P{GawB}qsmNP6258
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General Information
Symbol
Dmel\P{GawB}qsmNP6258
Species
D. melanogaster
Name
FlyBase ID
FBti0036879
Description
Inserted Element
LINE ID
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
Genomic Location
Chromosomal Location
2R ( 57A6 )
Sequence Location
2R:20,579,056..20,579,056 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:20,579,056..20,579,056 [-]
Orientation
Cytological location (computed by FlyBase)
57A6 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Size (Kb)

11.279

Component Allele(s)
Molecular map
Affected Gene(s)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

qsm reporter expression is observed in clock neurons. It is most strongly expressed in the 2 DN2, 4-17 DN3, 1-2 LNd, and 1 LNv neurons. Some but not all of the clock neurons that express qsm also express cry. 1-2 LNd and 1-2 of the DN3 neurons, 1 DN1 neuron, and the single fifth s-LNv neuron coexpress cry while 2 DN2 neurons and ~10 DN3 neurons are cry negative. Reporter expression is observed in wide neuronal arborizations covering an area of the antennal lobes, tritocerebrum, and suboesophageal ganglion. Ramifications in the the contralateral antennal lobes are interlinked by many short connectives and a prominent axonal bundle runs to the antennal nerve. Arborizations in the tritocerebrum and the suboesophageal ganglion are connected with nerves innervating the mouthparts and the ventral cord. A number of stained neurons are located along the antennal lobe-tritocerebrum-suboesophageal ganglion arborization. Neurons in the posterior pars intercerebralis and neurons in lateral and ventral parts of the suboesophageal ganglion are labelled. Signal is also seen in a bundle of ramified fibers that interlink the contralateral optic lobes passing through the lateral and dorsal brain. Neurons innervating this link are located in regions containing clock neurons. In the optic lobes, ramified fibers are visible in a middle layer of the medulla.

 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 2R:16093918-16093919 refined by FlyBase alignment of dbGSS accession AG219123 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{GawB}NP6258
P{GawB}NP6258-5-1
P{GawB}l(2)05510NP6258
P{GawB}qsmNP6258
qsm105-Gal4
Name Synonyms
Secondary FlyBase IDs
    References (7)