A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
The transcriptional mbl reporter is expressed in third instar larval photoreceptors. In the brain, expression is observed mainly in post-mitotic neurons with some expression in glial cells. An intersectional strategy was used to label single cells. mbl reporter is observed in L1, L4, R7 and R8 neurons. It is detected early in pupal development but not later. mbl is expressed in a cell-specific manner that correlates with the expression of Dscam2 exon 10B but not 10A. It is not detected in mushroom body neurons that express Dscam2 exon 10A in adults.
Location 2R:12846014-12846441 determined by FlyBase alignment of dbGSS accession AG215426 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.