FlyBase Insertion Report: Dmel\P{lacW}Noc2[k07215]

General Information

Symbol

Dmel\P{lacW}Noc2^k07215

Species

D. melanogaster

Name

FlyBase ID

FBti0006703

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k07215

Affected gene(s)

Ecol\lacZ, l(2)k07215, Noc2

Viability / fertility

Associated allele(s)

Ecol\lacZ^k07215, l(2)k07215^k07215, Noc2^k07215

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2L ( 39A7 )

Sequence Location

2L:21,163,661..21,163,661 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:21,163,661..21,163,661 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

39A7 (inferred by FlyBase from sequence location)

Cytological location (reported)

39B1-39B2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034159

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(BDGP Project Members, 1994-1999)

l(2)k07215

(BDGP Project Members, 1994-1999)

Noc2

(Kahsai et al., 2016)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^k07215

Phenotypic Class Phenotype Manifest in Detailed Description

l(2)k07215^k07215

Phenotypic Class Phenotype Manifest in Detailed Description

Noc2^k07215

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k07215

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10662

y¹ w^67c23; P{lacW}Noc2^k07215, l(2)k07215^k07215/CyO

Kyoto

102540

y¹ w^67c23 ; P{lacW}Noc2^k07215 / CyO

External Crossreferences and Linkouts ( 0 )

Comments

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion. The insertion maps within the CG9246 transcription unit. However, since the insertion lies within a gene-rich region, the lethality can not yet be conclusively mapped to a single gene from these results. Thus l(2)k07215^k07215 (representing the lethality of the chromosome) has been kept as a separate allele in FlyBase for now.

(Kahsai et al., 2016)

Location 2L:21151254-21151255 confirmed by FlyBase alignment of dbGSS accession AQ034159 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{lacW}CG9246^k07215

P{lacW}Noc2^k07215

P{lacW}l(2)k07215^k07215

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close