FlyBase Insertion Report: Dmel\P{GawB}bbg[C96]

General Information

Symbol

Dmel\P{GawB}bbg^C96

Species

D. melanogaster

Name

FlyBase ID

FBti0006245

Feature type

transposable_element_insertion_site

Also Known As

C96-GAL4, C96Gal4, C96, C96 Gal4, bbg-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

bbg, Scer\GAL4

Viability / fertility

viable

Associated allele(s)

bbg^C96, Scer\GAL4^bbg-C96

Stock availability

8 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

(Kim et al., 2006)

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Doc

(Kim et al., 2006)

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

bbg

(Kim et al., 2006)

Scer\GAL4

(Jones et al., 2012, Zhang et al., 2012, Zhang et al., 2012, Ni et al., 2011, Olson et al., 2011, Bahadorani et al., 2010, Boyles et al., 2010, Chen et al., 2010, Hermle et al., 2010, Herr et al., 2010, Kleinschmit et al., 2010, Mesquita et al., 2010, Saj et al., 2010, Usha and Shashidhara, 2010, Ni et al., 2009, Shalaby et al., 2009, Gupta et al., 2008, Ji et al., 2008, Ni et al., 2008, Kankel et al., 2007, Li et al., 2007, Pepple et al., 2007, Alexander et al., 2006, Childress et al., 2006, Jafar-Nejad et al., 2006, Kim et al., 2006, Rives et al., 2006, Seto and Bellen, 2006, Anderson et al., 2005, DasGupta et al., 2005, Hayward et al., 2005, Krupp et al., 2005, Mukherjee et al., 2005, Schubiger et al., 2005, Tsuda et al., 2005, Baeg et al., 2004, Duman-Scheel et al., 2004, Quinn et al., 2004, Butler et al., 2003, Enerly et al., 2003, Jafar-Nejad et al., 2003, Johnston and Sanders, 2003, Marygold and Vincent, 2003, Settle et al., 2003, Srivastava and Bell, 2003, Duman-Scheel et al., 2002, Presente et al., 2002, Xu et al., 2002, Xu et al., 2002, Yedvobnick et al., 2002, Baeg et al., 2001, Dietrich et al., 2001, Nolo et al., 2001, Stewart et al., 2001, Yedvobnick et al., 2001, Helms et al., 1999, Lin and Perrimon, 1999, Johnston and Edgar, 1998, Kim and Boulianne, 1998, Yaich et al., 1998, Cohen et al., 1997, Gustafson and Boulianne, 1996, Ludlow et al., 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

bbg^C96

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^bbg-C96

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

abdominal sensory neuron

(Kim et al., 2006)

pharyngeal muscle cell

(Kim et al., 2006)

embryonic/larval salivary gland

(Kim et al., 2006)

mechanosensory neuron of embryonic/larval posterior spiracle

(Kim et al., 2006)

mechanosensory neuron of embryonic telson

(Kim et al., 2006)

larval stage

embryonic/larval central nervous system

(Kim et al., 2006)

third instar larval stage

dorsal-ventral compartment boundary of the wing disc

(Usha and Shashidhara, 2010)

wandering third instar larval stage

presumptive wing margin

(Kim et al., 2006, Gustafson and Boulianne, 1996)

mechanosensory neuron of wing hinge primordium

(Kim et al., 2006)

dorsal-ventral compartment boundary of the haltere disc

(Kim et al., 2006)

eye disc posterior to the morphogenetic furrow

(Kim et al., 2006)

presumptive second segment of antenna | restricted

(Kim et al., 2006)

antennal disc | restricted

(Gustafson and Boulianne, 1996)

dorsal-ventral compartment boundary of the wing disc

(Srivastava and Bell, 2003)

oogenesis

polar follicle cell

Comment: early stages

(Aranjuez et al., 2012)

oogenesis stage S9 -- S10

border follicle cell

(Aranjuez et al., 2012)

follicle cell

(Aranjuez et al., 2012)

Additional Information

Statement

Reference

Drives expression in a broad band at the presumptive wing margin of the wing disc.

(FlyBase Curators, 2017)

Expression is seen in anterior and posterior polar cells at early stages in the egg chamber. By stages 9 and 10, expression is found in the majority of follicle cells including border cells.

(Aranjuez et al., 2012)

In the late embryo, ScerGAL4^bbg-C96 drives expression in sensory neurons of the abdomen, including in sensory organs associated with the posterior spiracles and the telson; in muscle cells of the developing pharynx, and in the salivary glands. ScerGAL4^bbg-C96 drives broad expression in the larval central nervous system. ScerGAL4^bbg-C96 drives expression in a broad stripe at the presumptive wing margin in the wing disc, and in cells that correspond to the future sense organs of ventral radius of the wing hinge; in a similar pattern in the haltere disc; in a punctate pattern posterior to the morphogenetic furrow in the eye disc; and in several specific cells within the presumptive second segment of the antennal disc. There is no significant expression in the leg discs.

(Kim et al., 2006)

ScerGAL4^bbg-C96 drives expression along the D/V boundary in the wing disc.

(Srivastava and Bell, 2003)

In third instar larval eye-antennal disc, expression in a small group of cells in the posterior of antennal disc. In the wing disc, expression in wing margin precursor cells.

(Gustafson and Boulianne, 1996)

Marker for

Reflects expression of

bbg

(Aranjuez et al., 2012, Usha and Shashidhara, 2010, Kim et al., 2006)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Aranjuez et al., 2012)

Ecol\lacZ^UAS.cBa

(Kim et al., 2006)

Data on Genetic Line

Line ID