FlyBase Insertion Report: Dmel\P{lacW}Pol-1

General Information

Symbol

Dmel\P{lacW}Pol-1

Species

D. melanogaster

Name

FlyBase ID

FBti0003949

Feature type

transposable_element_insertion_site

Also Known As

omb-lacZ, P{lacW}bi^Pol-1, omb^P1

Description

Inserted Element

P{lacW}

LINE ID

Affected gene(s)

bi, Ecol\lacZ

Viability / fertility

viable

Associated allele(s)

bi^D13-1, Ecol\lacZ^bi-Pol-1, Ecol\lacZ^D11, w^Pol-1

Stock availability

None publicly available

Genomic Location

Chromosomal Location

X ( 4C3 )

Sequence Location

X:4,411,468..4,411,475 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:4,411,468..4,411,475 [+]

(Tsai et al., 1997)

Orientation

Cytological location (computed by FlyBase)

4C3 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

(Grimm and Pflugfelder, 1996)

Ecol\lacZ

(Fischer et al., 2012, Svendsen et al., 2009, Murakami et al., 2007, Ramel et al., 2007, de Navas et al., 2006, Adachi-Yamada et al., 2005, Thompson et al., 2005, Bharathi et al., 2004, del Alamo Rodriguez et al., 2004, Winter and Campbell, 2004, Bennett et al., 2003, Muller et al., 2003, Adachi-Yamada and O'Connor, 2002, Hasson et al., 2001, Certel et al., 2000, Sivasankaran et al., 2000, Wang et al., 2000, Adachi-Yamada et al., 1999, Campbell and Tomlinson, 1999, Goto and Hayashi, 1999, Minami et al., 1999, Zilian et al., 1999, Abu-Shaar and Mann, 1998, Gonzalez-Crespo et al., 1998, Huang and Kunes, 1998, Wisotzkey et al., 1998, Jackson et al., 1997, Tsai et al., 1997, Tsuneizumi et al., 1997, Brook and Cohen, 1996, Gonzalez-Crespo and Morata, 1996, Grimm and Pflugfelder, 1996, Zecca et al., 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

bi^D13-1

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^bi-Pol-1

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^D11

Phenotypic Class Phenotype Manifest in Detailed Description

w^Pol-1

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Sun et al., 1995)

embryonic stage 8

procephalic segment

(Tsai et al., 1997)

embryonic stage 12

adult optic lobe

(Tsai et al., 1997)

embryonic stage 12 -- 14

antennal segment

(Tsai et al., 1997)

central nervous system | segmentally repeated

(Tsai et al., 1997)

embryonic stage 13

antenno-maxillary complex

(Tsai et al., 1997)

larval stage

embryonic/larval salivary gland

(Lemaitre and Coen, 1991)

embryonic/larval Malpighian tubule

(Lemaitre and Coen, 1991)

embryonic/larval brain

(Lemaitre and Coen, 1991)

second instar larval stage -- wandering third instar larval stage

wing pouch | medial

(del Alamo Rodriguez et al., 2004)

early third instar larval stage -- wandering third instar larval stage

wing hinge primordium | medial

(del Alamo Rodriguez et al., 2004)

pupal stage

wing hinge

(del Alamo Rodriguez et al., 2004)

wing blade

(del Alamo Rodriguez et al., 2004)

Additional Information

Statement

Reference

EcollacZ^bi-Pol-1 is expressed in a stream of cells migrating from the proximal to the distal part of the inner proliferation zone in the larval optic anlage.

(Apitz and Salecker, 2015)

EcollacZ^bi-Pol-1 is expressed in a wide band centered on the A/P boundary in the wing pouch and wing hinge primorium areas of the wing disc.

(del Alamo Rodriguez et al., 2004)

Expression of theP-element EcollacZ insertion line reflected by X-gal staining, closely mirrorsexpression of bi transcripts. In the stage 8 embryo, expression is seenanterior to the cephalic furrow in lateral clusters. In stage 12 embryos,expression in the optic lobe and the antennal segment is high and persiststhrough embryogenesis. From stage 12 through stage 17 expression is detected ina segmentally repeated pattern in the ventral nervous system. EcollacZ expressionis also detected during larval development in the brain and the imaginal discs.Expression is high in the dorsal and ventral marginal regions of theeye-antennnal disc, in a broad stripe in the wing pouch area of the wing disc,and along the dorsal anterior-posterior boundary in the leg disc. Expression isalso high in the optic lobe of the larval brain.

(Tsai et al., 1997)

In third instar wing disc, expressed in the wing pouch and in the presumptive hinge region. Expression does not occur throughout the wing pouch; symmetrical relative the the D-V boundary and the A-P boundary.

(Grimm and Pflugfelder, 1996)

In embryo, "patterned" expression.

(Sun et al., 1995)

Marker for

Reflects expression of

(Apitz and Salecker, 2015, del Alamo Rodriguez et al., 2004, Tsai et al., 1997, Grimm and Pflugfelder, 1996, Sun et al., 1995)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

The 8bp insertion associated host repeat is indicated. The P insertion is in a negative orientation with respect to bi gene transcription.

(Tsai et al., 1997)

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

Bi-lacZ

(Bennett et al., 2003)

Omb-LacZ

(Campbell and Tomlinson, 1999)

Pol-1

(Sun et al., 1995)

P{lacW}Pol-1

(Sun et al., 1995, FlyBase, 1992-)

P{lacW}bi^Pol-1

(Tsai et al., 1997, Grimm and Pflugfelder, 1996, FlyBase, 1992-)

bi^P1

(Peterson and O'Connor, 2013)

omb-Z

(Hasson et al., 2001)

omb-lacZ

(Apitz and Salecker, 2015, Tsai et al., 2015, Peterson and O'Connor, 2013, Fischer et al., 2012, Svendsen et al., 2009, Ramel et al., 2007, de Navas et al., 2006, Thompson et al., 2005, Bharathi et al., 2004, Winter and Campbell, 2004, Certel et al., 2000, Wang et al., 2000, Goto and Hayashi, 1999, Zilian et al., 1999)

omb-lacZ^P1

(Adachi-Yamada and O'Connor, 2002)

omb^P1

(Tsai et al., 2015, Murakami et al., 2007)

omb^P1-lacZ

(del Alamo Rodriguez et al., 2004)

ombZ

(Muller et al., 2003)

Name Synonyms

Secondary FlyBase IDs

FBti0003762

References (39)

Report Sections

Open Close