FB2024_03 , released June 25, 2024
Sequence Feature: Dmel\dsRNA-GD10304
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General Information
Symbol
Dmel\dsRNA-GD10304
Species
D. melanogaster
Feature type
FlyBase ID
FBsf0000083285
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:21,493,512..21,493,811 [+]

2L:21,453,023..21,453,322 [+]

2L:21,433,934..21,434,233 [+]

2L:21,423,826..21,424,125 [+]

2L:21,473,230..21,473,529 [+]

2L:21,468,187..21,468,486 [+]

2L:21,463,144..21,463,443 [+]

2L:21,458,084..21,458,383 [+]

2L:21,478,273..21,478,572 [+]

2L:21,488,467..21,488,766 [+]

2L:21,498,557..21,498,856 [+]

2L:21,483,316..21,483,615 [+]

2L:21,503,610..21,503,909 [+]

2L:21,533,273..21,533,572 [+]

2L:21,513,699..21,513,998 [+]

2L:21,538,317..21,538,616 [+]

Sequence Data
Length
300
Comments

One copy of repeat shown

AGGCAAAGTCCCGCTCAAACCGTGCCGGTCTTCAATTCCCTGTGGGCCGTATTCACCGTTTGCTCCGGAAGGGAAACTAC
GCAGAGCGTGTTGGTGCAGGCGCTCCAGTTTACCTAGCTGCCGTAATGGAATATCTGGCCGCTGAGGTTCTCGAGTTGGC
TGGCAATGCTGCTCGTGACAACAAGAAGACTAGAATTATTCCGCGTCATCTGCAACTGGCCATCCGCAACGACGAGGAGT
TAAACAAGCTGCTCTCCGGCGTCACAATTGCACAAGGTGGCGTGTTGCCTAATATACAGG
Associated Information
Experimental Data
Progenitors
PCR template

genomic DNA

Primer 1
AGGCAAAGTCCCGCTCAAACC
Primer 2
CCTGTATATTAGGCAACACGCCACCT
Comments
Collection Information
Collection: VDRC-GD
Symbol
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC first generation.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Vector of progenitor construct
Description

Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.

NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

Additional data
Sample preparation
Collection preparation

A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.

Mode of assay
Data analysis

A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.

Cell lines
Observed in
Not observed in
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
dsRNA-GD10304
Secondary FlyBase IDs
  • FBri0076725
References (2)