The following split-GAL4 combinations were generated by Kei Ito and Masayoshi Ito in association with Gerry Rubin at Janelia: The SS21077 split-Gal4 is a combination of VT017413-AD (attP40) and GMR82F01-DBD (attp2). The SS21206 split-Gal4 is a combination of GMR68A10-AD (attP40) and VT007780-DBD (attp2). The SS21208 split-Gal4 is a combination of GMR65D05-AD (attP40) and GMR45D04-DBD (attp2). The SS24252 split-Gal4 is a combination of VT012596-AD (attP40) and GMR83D12-DBD (attp2). The SS24588 split-Gal4 is a combination of GMR25G10-AD (attP40) and GMR56B05-DBD (attp2). The SS31827 split-Gal4 is a combination of GMR25G10-AD (attP40) and GMR27H01-DBD (attp2). The SS27580 split-Gal4 is a combination of GMR45F09-AD (attP40) and GMR70E08-DBD (attp2). The SS27978 split-Gal4 is a combination of GMR41H07-AD (attP40) and GMR65F12-DBD (attp2). The SS28308 split-Gal4 is a combination of GMR35C12-AD (attP40) and GMR17A10-DBD (attp2). The SS31522 split-Gal4 is a combination of GMR89B06-AD (attP40) and GMR49A06-DBD (attp2). The SS32151 split-Gal4 is a combination of GMR51D07-AD (attP40) and GMR48D11-DBD (attp2). The SS35902 split-Gal4 is a combination of VT014340-AD (attP40) and GMR68A08-DBD (attp2). The SS36651 split-Gal4 is a combination of VT043699-AD (attP40) and GMR11A07-DBD (attp2). The SS39070 split-Gal4 is a combination of VT065322-AD (attP40) and GMR83H09-DBD (attp2). The SS39616 split-Gal4 is a combination of VT050106-AD (attP40) and GMR78H08-DBD (attp2). The SS42968 split-Gal4 is a combination of GMR31H05-AD and GMR77F03-DBD. The SS42983 split-Gal4 is a combination of GMR83H09-AD (attP40) and GMR68B03-DBD (attp2). The SS46337 split-Gal4 is a combination of VT037550-AD (attP40) and VT046831-DBD (attp2). The SS46613 split-Gal4 is a combination of GMR12D12-AD and GMR11D02-DBD. The SS49941 split-Gal4 is a combination of VT026839-AD (attP40) and VT062645-DBD (attp2). The SS51316 split-Gal4 is a combination of VT049245-AD (attP40) and VT013121-DBD (attp2). The SS49970 split-Gal4 is a combination of GMR78E09-AD (JK22C) and VT062645-DBD (attp2). The SS27959 split-Gal4 is a combination of GMR48A08-AD (attP40) and GMR60F09-DBD (attp2). The SS28305 split-Gal4 is a combination of GMR33G04-AD (attP40) and GMR26B04-DBD (attp2). The SS45221 split-Gal4 is a combination of VT018689-AD (attP40) and VT029309-DBD (attp2). The SS45251 split-Gal4 is a combination of VT022004-AD (attP40) and VT045575-DBD (attp2). The SS46348 split-Gal4 is a combination of VT000877-AD (attP40) and VT046831-DBD (attp2). The SS47568 split-Gal4 is a combination of GMR29B04-AD (attP40) and VT041692-DBD (attp2). The SS52046 split-Gal4 is a combination of GMR9F06-AD and VT013602-DBD. The SS52050 split-Gal4 is a combination of VT057286-AD (attP40) and VT008170-DBD (attp2).