FB2024_03 , released June 25, 2024
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Pagan, E.A., Orr-Weaver, T. (2020.11.30). Location data for nebu[7] deletion. 
FlyBase ID
FBrf0247377
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication 
The nucleotide sequence of the deleted region for nebu7 (based on the design on the gRNAs and donor plasmid) is below.
The deleted region corresponds to  3L:12825753..12832109-. The location and size of the deletion were confirmed by both PCR and sequencing.
nebu7 deleted genomic sequence
5’-CCATCGTAACTGTGCAATCCTACCGAAGTTTACATAATCCCTTCGATATGTCCACCGCATCGTCGAGCAACTCCAGCGGCGAGGAGGCCAACAAAAAGTCCGGCTCCAACAACGAGGAGAGCTCCCACCTGCTGGAATCCGCCGAAAATGGAGCCAAGTACGGCTCCAGACAGCCGGTCAAAATCGTCAAAGCCAGCGATAATGGACGCACCAGCTCCACACCACATgtacgcaaagattcgcatgttttcatagataagagcactgagaaaaataagacatatataagtttgaaattttgcaaattatgattagttgaaacttatgtttaaagaaacaaactatttctactacgctcagagcctaaagacttaatcgttaattatttcttgtgctctgtatttatgtttttttctcagtgcttgcttaactttagttaattaacgctgtccagctggttagccaagttataatgcatcttttgatgttgaccttggctgagagaccacggcgaaagttcaattaacgcagtgtgaaaaacgagacaggaaataaagttgcttaagcagcaaatatacaaatgccctcaatataaaataatcaagttgtgatgcctttccaatgtaagggcattaaaaatctgcgttgtagctccaaatatttgctcaaaataggcaaacaatctttgttgttgcagaagtaaatgaggggctgaaagcagaaagatgttgccgctaaaagatacgagatataaatagtttaccaggcaattatcaggctgaaatgagtatttttccaattccaaaagctatctctatcaagcgagagcacaaacaaagaaaatagagtataatttgccttttctgaaaccgcacgagttcacataccttatccgcttaacatacatacatgtattttaagcgataaacaatgaaaacacaataatgtttttcagtggactttatcttaaatccagtacgttttgctacattctggtagctaagcgttcattgatggatcgcacagcttatcaaactaatgagttccgttccaaaaacaatgtggaaactttctatttttatttggttttatgttgaacatggtcacaataggcgaacaaatgctatgaaatacaaatcattacctatagaaaattaattttgccttgcaaagaataatgtgataagtgagcacttcaaaaataggggcgtattcccgattccccgaaagtagaaactagaaataacaatggtcacaatacggttttgttttggaataaccgaaaccatacgtacatatatgaattttatcagacaaattcaattgaaactcgttccactgaattcccaaaatgagtcgacaattttgtgggtgtttattttagtacaaagaacacacgcattaaataaatttgaatttgaatacactgaattcccaaaatgagtcaacaattttgtgggtgtttattttagtacaaagaacacacgcattaaataaatgcgacacccctttaactctaaaaagaaatgcacttatatagacactggaatgattttatgactaccatttgaatacgttttaaaatgcgacccacaaaattataaaaaactcggccagttcgataatgaagtgtggcacattttttgaatatcaatgagttgccctaaatggttcgctatgatggttataaagccatcaccgttttcactttcattgtcatcgtaatacacaccaaattaagccattataaaacaactttgaatagttttgagtataatattgaaagtagcacgatatactcaaggccttagaggaattattcaaaaaaagatgggtgcacatttattaaggtattttaaaaaccaaataatcttaataataagcactgtttccataaatacgcgtaaaatgtagtaggaatttttcgaactatttttatgcaaattttatgagcaatagcccgggattattgtgcatatattaacctagaccactcaataattatggagtatttaagcggaattggcttatgctctacagttaaccagtttagaggaatgccaaaaggcatattaaattaccattcttacttatgacgcgcactgttgcgcctgccactgacattgaaacgaacttgaacctccgcccatttggccagtatcatcaacgggtttgcgggggcggtgggacttagcacccgaaagtgggggactttaactggcagtaatactaattttagatacagaacgacaggaaggcactcagacaggcaatggattgaccacattagccgcagacggaactcaaacaaaccaaggcacagaatcgtggacagatatcgatttcagacgcgagatttgaacgcttcgcttagtcgcgactggcaatctggaggtgtgtcacccagtcgaccagagcaaagtgacagttaatatgtgggcgtatatgtacatatacgcctgtgctgttgccacatggccttctcctttttccagctgcaaatcttggcgtgcattttcacggaagtgatttgttctctgctccttcgtttcgcgtgggtcgccagattggcaatggaactttgagtgggattcgccagtgggcggcgacacatgagatgaggcaagttataggcgacgaagaaatagccataaattacccatatgcgcgaaatggcttttacgatggtctcttagacatgcaatatgcaataagcgaaaatattatgataatgagcgctattgtatctaagatctgtaaagaaatccatttgaaaatctctaaagaaatccatttaaaaatctctaaagaaatccatttagaaaaaaaaaagcatttcacctcgatttagttcaacaattattacttattttcaaatggaatttacgctatgctacagaacaacaaaatatattgtgtagatgtttccaatgtaaaaacaagctttgaatttgttgaaaacaattcgaacaggcccccttttttcgcaaaaaaaaatgtacaaatcacgtacaacacgtgccaaaagaaaagtaagaggatatgtaagatacatttaggttactttggtctattgactatattcagaatttaatttgaacaactatataatatttatgtttctataagttagtgaaaatatgtttatatgtttagaataccgtgtcataggtcaagtttacttaaaatagtatctaatcattcatgaaacacgccccttttaagcacagaaaataaagcacaaccgcatattgttctgtttataaacatgaaaatatcttaaaaaagcttttgataaataaaagctgcgctgaatgccggtcggctatattcttaaacacgtttatagatggaatttgggcaacttgctgattaggcacgcgataacctaagccaaatgtacacatatatatataggccgatttcacggtaatttatggccaatgaacggaagtcaatgagtatggttaacaatgtggtgaaaacactacatatatgagtagttaattggtgctgcttgagtgatctcaacaccttgactagtggagtaaaataaaaggggaatgtatactatcttaagaaatatctataggtagaatggatttgaagtgagtggctaaggggttattttgccatgatgtttatctttaatgcggcttaaattattacgtgtagctattggtaaatgtatgtagattagaactctatatatatccatcgtttaattaggtaatttccatttacttaatctccgccttatctacgaaactctatcagcttatgcttataagtacaagaagtttcattgatcaagtgccggtcttaaaaacattcaagtggaaacacatggacataataatagtactttgcgtttcaactttaaaacttcccatgcggcatccctaacttcaaatatacccaccgatttctcgggtgtcttatgggattttcctgtccatataagcttgctgacccggaccactcggcgtatgaatgatattcagtggcgagtgctcgcgaacctgccttactttgggggctacttctgtttatgtgactggtataatgaaataatgaagcacttgagcccaatcggaatgggaagagcatagccatggccaccggttactttttgtatatattttttggggccgagagagtgcaataaatactatcggacctccaataaatgtcctaatcactgagcgggggttctgaccttatcaagaaccatggcatgataatacttgtaggtagctaatagcTGGCCCCCAGTCTAAGTCAGTTGGTCAGAGCAGAAAGATCTAATTAAAAGTGAAAAgtaatgtattatgtaaaataaggatatatgtatatttaattaattttcattgtgaatcggtttgaacgaaatatgttgatcaaggacaatgatcgtgttgctcattaatcttaaatacttcttatagaagtattaaacgaatatgcaatattattaatttaggccagtatttacaatttacttatcaaatccttcaaccattgcagGATCAGGAGAATCTACTGGGCAACATGGTGCACACGTCTACTTCGAATAACTCCCAGAAGTGGCCACAATATGTGGCCGCATTGGCCGCTGCTGGTGGCGCCTTCGCCGCCGGTACCGTCCTGGGTTGGACATCGCCGGCCGAAACGGAGATCGTGGATCGCGGCGAAGGATACGATTTCCCTGTCGACAAGGATCAGTTTTCGTGGGTAGGATCTGCCATGACCCTGGGTGCCGCCTGTGTGTGCATTCCCATTGGCTTCCTGATCAACATGATTGGCCGAAAGTGGACCATGCTGTTTCTGGTGCTGCCCTTCATTCTCGGTTGGACCATGCTCATCTGGGCGGTCAATGTCAGTATGCTGTATGCCTCGCGCTTCATCCTCGGTATCGCCGGTGGAGCTTTCTGTGTGACCGCTCCCATGTACACGGGTGAGATTGCTCAGAAGGAGATTCGCGGAACGCTGGGCAGCTTCTTCCAGTTGATGATAACCATTGGTATCCTGTTCGTCTATGCCGTCGGAGCTGGTGTGAAAATATTCTGGTTGAGCATCATTTGCGGCATTCTGCCCCTCATCTTTGGCGCCATCTTCTTTTTCATGCCGGAATCGCCCACCTACTTGgtaagttttatagatcttcagttccacaacaaattttaaagccccaatacaatattatatattatatgaacattttatattatgtatgtagtagcattataatattatatattattttcgaaaaaaaacaccctgtaaattaatatctcaaatggcgcactataaaattaatccattggtcagatataatctgccagtcaatcaagattaaatcttgggcaaacccaccgtttatatctaaaagatacgaaaccaccacgagtttgccatcaaaaccagaaattccttttcaagtgtaatgcaacatgtccaatttccaaatccccatcgacagGTTTCCAAAGATCGGTCGGAGAACGCCATCAAGTCCATTCAATGGCTGCGTGGCAAGGAATACGATTACGAGCCAGAGCTGGCGGAGCTGCGTGAAACCGACCGGGAAACCAAGGCCAACAAGGTCAACGTGTGGGCTGCTCTGAACCGACCCGTCACCCGGAAGGCTTTGGCCATCAGCATGGGTCTTATGTTCTTCCAGCAGGTCTGCGGAATCAATGCTGTTATTTTCTACGCTTCGAGGATCTTTTTGgtgagtgtgaatttgattgttttagaatctcaaaaataaatcaataattattacattccctccaattcagGAAGCCAACACTGGCATCGAGGCTGAGTGGGCAACGATCCTGATCGGTATCATGCAAGTGGTGGCCACCTTCGTCTCCACCCTGGTAGTAGACAAGCTGGGCCGTCGCATCCTGCTGTTGGCCTCAGGAATTTCCATGGCCATTTCCACCACCGCCATCGGAGTGTACTTCTTCCTCCAGAAACAGGACGCGGCACAAGTGGTGAGTCTGGGCTGGCTCCCAGTGGCCAGTCTGTGCCTGTTCATCATCATGTTCTCGATCGGCTATGGACCGGTGCCATGGCTGATGATGGGAGAGCTCTTCGCCACCGACATCAAGGGATTTGCTGGATCGCTGGCCGGAACATCCAACTGGCTTTTGGCATTCGTGGTGACCAAGACGTTTGTGAACTTGAACGATGGCCTGGGAATTGGCGGAACCTTCTGGCTATTCGCTGGTCTGACTGTTGTGGGTGTGATATTCGTTTATTTCGCCGTGCCGGAAACCAAGGGCAAGAGTCTCAACGAGATTCAGCAGGAGCTGGCTGGCAACCGGTCGACTCCACAAGCTATTC-3’
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Research paper

Identification of New Regulators of the Oocyte-to-Embryo Transition in Drosophila.
Avilés-Pagán et al., 2020, G3 (Bethesda) 10(9): 2989--2998 [FBrf0246611]

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