The mutant alleles of Oa2, Octbeta2R, Octbeta3R, Oct-TyrR and TyrRII that are used in sleep analysis experiments in Figures 6, S7 and S8 of Deng et al., 2019, Neuron 101(5): 876--893.e4 <up>FBrf0241737</up> were generated using ends-out homologous recombination to replace the coding sequence of the first coding exon with Gal4.