The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Annie Handler and Vanessa Ruta, The Rockefeller University. PBac{UAS-ER.GCaMP6-150} is composed of an ER-localized calcium sensor, GCaMP6, under the control of UAS (built in the pJFRC81 vector). GCaMP6 is fused N-terminally to the human calreticulin signal peptide and C-terminally to the KDEL ER-retention signal. This particular variant has a 150 mM affinity (Kd) for Ca2+. Details on the mutations in GCaMP6-150 compared to GCaMP6 can be found in de Juan Sanz et al., 2017. Neuron. 93(4)867. PBac{UAS-ER.GCaMP6-150}VK00005 is an insertion on the third chromosome in an attP site at 75A10, 3L:17952108 .