FB2024_03 , released June 25, 2024
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Ruta, V. (2019.8.14). PBac{UAS-ER.GCaMP6-150} construct and insertion. 
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FBrf0243245
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Annie Handler and Vanessa Ruta, The Rockefeller University.
PBac{UAS-ER.GCaMP6-150} is composed of an ER-localized calcium sensor, GCaMP6, under the control of UAS (built in the pJFRC81 vector). GCaMP6 is fused N-terminally to the human calreticulin signal peptide and C-terminally to the KDEL ER-retention signal. This particular variant has a 150 mM affinity (Kd) for Ca2+. Details on the mutations in GCaMP6-150 compared to GCaMP6 can be found in de Juan Sanz et al., 2017. Neuron. 93(4)867.
PBac{UAS-ER.GCaMP6-150}VK00005 is an insertion on the third chromosome in an attP site at 75A10,  3L:17952108 .
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    English
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    Alleles (1)
    Genes (1)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (4)
    Transgenic Constructs (1)