The following information accompanied stocks donated to the Bloomington Stock Center by Eunice Chan, Aix Marseille Université. P{UASp-sqh.E20E21} was constructed in the pP{UASp} vector in the laboratory of Roger Karess, Université Paris Diderot. It expresses a phosphomimetic sqh protein with T20E and S21E substitutions under UAS control. The mutated sqh coding sequences are the same as those present in P{sqh-E20E21} (http://flybase.org/reports/FBtp0014087.html). P{UASp-sqh.E20E21}3 is a third chromosome insertion.