FB2024_03 , released June 25, 2024
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Nybakken, K. (2014.2.28). P{UAS-wdb.HA.N} and P{UAS-wdb.95-524.HA} constructs and insertions. 
FlyBase ID
FBrf0224303
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Stock Center by Kent Nybakken, Boston Biomedical Research Institute.
P{UAS-wdb.HA.N} was generated by PCR amplifying the coding region of wdb (FBgn0027492) from the LD34343 cDNA with primers that incorporated a 5' Bgl II site and a 3' Not I site. This PCR fragment was then ligated into a vector made by Kent Nybakken containing an in-frame, C-terminal, triple hemagglutinin (HA) tag followed by a stop codon. The tagged form of wdb was then excised using Bgl II and Xba I and cloned into identically cut pP{UAST}.
P{UAS-wdb.95-524.HA} was generated similarly by amplifying the coding region for amino acids 95 through 524. The resulting protein acts as a dominant negative.
P{UAS-wdb.HA.N}3M and  P{UAS-wdb.95-524.HA}1M are second chromosome insertions.
P{UAS-wdb.HA.N}5M and P{UAS-wdb.95-524.HA}6 are third chromosome insertions.
--
Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
http://flystocks.bio.indiana.edu
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    English
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    Alleles (2)
    Genes (1)
    Natural transposons (1)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (2)