From: Kevin Cook <kercook@XXXX> Date: 15 June 2009 13:12:49 BST To: FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchrist@XXXX> Subject: Isolation and characterization of Df(3L)BSC807 Isolation and characterization of Df(3L)BSC807 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC807 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03852 and PBac{RB}CG7375e02842. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d03852/PBac{RB}CG7375e02842 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC807 from the segment of P{XP}d03852 to the left of its FRT site and the segment of PBac{RB}CG7375e02842 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(3L)BSC807 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3L:7643513 ;8190699 and the cytological breakpoints predicted from these coordinates are 66A8;66B12. Df(3L)BSC807 failed to complement Pdp1P205 and pbl3. -- Kevin Cook, Ph.D Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu
