From: kercook@XXXX
Subject: Isolation and characterization of Df(3L)BSC730
Date: 28 February 2009 17:33:18 GMT
Isolation and characterization of Df(3L)BSC730
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC730 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00991 and P{XP}d03775. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f00991/P{XP}d03775 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC730 from the segment of PBac{WH}f00991 to the left of its FRT site and the segment of P{XP}d03775 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC730 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 68F7;69E6. Df(3L)BSC730 failed to complement eyg1, Ptp69D1 and Atg100305.
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Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
kercook@XXXX
812-856-1213 (office), 812-855-2577 (fax)
http://flystocks.bio.indiana.edu
