From: kcook@XXXX
Subject: Isolation and characterization of Df(1)BSC724
Date: December 28, 2008 1:20:03 PM GMT- 05:00
Isolation and characterization of Df(1)BSC724
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC724 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG15766d06145 and PBac{RB}e02418. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 P{XP}CG15766d06145/w1118 PBac{RB}e02418; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC724 from the segment of P{XP}CG15766d06145 to the left of its FRT site and the segment of PBac{RB}e02418 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(1)BSC724 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 5C2;5D3. It failed to complement rux2 and fs(1)M3K1.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX 
