From: kcook@XXXX
Subject: Isolation and characterization of Df(3R)BSC686
Date: December 28, 2008 1:15:51 PM GMT- 05:00
Isolation and characterization of Df(3R)BSC686
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC686 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00547 and P{XP}d05341. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f00547/P{XP}d05341 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC686 from the segment of PBac{WH}f00547 to the left of its FRT site and the segment of P{XP}d05341 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d05341 to be Release 3 genomic coordinate 23720672 on chromosome arm 3R. This corresponds to 98B5 on the Release 3 and Release 5 genome map. The predicted position of PBac{WH}f00547 on the Release 5 map is 98A1. Consequently, the cytological breakpoints of Df(3R)BSC686 are predicted to be 98A1;98B5. Df(3R)BSC686 failed to complement Df(3R)ED6265 and raps193.
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Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX 
