From: kcook@XXXX Subject: P{UASp-YFP.Rab27.Q74L}w07 Date: October 21, 2008 1:37:43 PM GMT- 04:00 To: flybase-cambridge@XXXX Cc: kschulze@XXXX Hi-- We've encountered a problem with the insertion P{UASp-YFP.Rab27.Q74L}w07 (http://flybase.org/reports/FBti0100864.html). The insertion maps to chromosome 2 based on segregation of the miniwhite marker from a second chromosome balancer in crosses. It's likely that the mapping of the insertion to the w gene based on flanking sequence came from some sort of rearrangement of the construct upon transposition. We have no information about the expression of Rab27 protein. Please make the appropriate changes to the insertion entry and rename the insertion P{UASp-YFP.Rab27.Q74L}07. Thanks! Kevin __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX