From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(3R)BSC611
Date: 	Wed, 03 Sep 2008  12:00:47  -0400  ( 17:00  BST)
Isolation and characterization of Df(3R)BSC611
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC611 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG9312[f04951] and P{XP}rdx[d06943]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C,Sb[1] females crossed to 
P{hsFLP}1, y[1] w[1118]; PBac{WH}CG9312[f04951]/P{XP}rdx[d06943] 
males. The males were heat shocked as larvae as described in Parks et 
al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC611 from the segment of PBac{WH}CG9312[f04951] to 
the left of its FRT site and the segment of P{XP}rdx[d06943] to the 
right of its FRT site. The cytological breakpoints of Df(3R)BSC611 
predicted from the Release 5 genomic coordinates of the transposable 
element insertions sites are 87F13;88A4. Df(3R)BSC611 failed to 
complement Nsf2[A6] and Df(3R)Exel6288.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX