From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC548
Date: 	Wed, 11 Jun 2008  14:30:25  -0400  ( 19:30  BST)
Isolation and characterization of Df(3R)BSC548
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC548 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d08662 and PBac{WH}CG1234[f00646]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}d08662/PBac{WH}CG1234[f00646] males. The males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC548 from the segment 
of P{XP}d08662 to the left of its FRT site and the segment of 
PBac{WH}CG1234[f00646] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC548 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 84C8;84D3. Df(3R)BSC548 failed to complement sas[15] and lap[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX