From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(1)BSC545
Date: 	Wed, 11 Jun 2008  14:30:21  -0400  ( 19:30  BST)
Isolation and characterization of Df(1)BSC545
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC545 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d05563 and PBac{WH}mew[f07434]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d05563/PBac{WH}mew[f07434]; MKRS, P{hsFLP}86E/+ females crossed 
to Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC545 from the segment of P{XP}d05563 
to the left of its FRT site and the segment of PBac{WH}mew[f07434] to 
the right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(1)BSC545 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
11C2;11E5. It failed to complement sno[71e1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX