From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC525
Date: 	Wed, 11 Jun 2008  14:29:46  -0400  ( 19:29  BST)
Isolation and characterization of Df(3R)BSC525
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC525 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}MTA1-like[d09140] and PBac{WH}CG2017[f01712]. 
The deletion was isolated as a chromosome lacking miniwhite markers 
in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed 
to P{hsFLP}1, y[1] w[1118]; 
P{XP}MTA1-like[d09140]/PBac{WH}CG2017[f01712] males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC525 from the segment of 
P{XP}MTA1-like[d09140] to the left of its FRT site and the segment of 
PBac{WH}CG2017[f01712] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC525 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 83B7;83C5. Df(3R)BSC525 failed to complement sec23[j13C8] 
and cas[j1C2].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX