From: Kevin Cook <kcook@XXXX> To: flybase-updates@XXXX Cc: Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(2L)BSC521 Date: Tue, 15 Apr 2008 15:32:44 -0400 ( 20:32 BST) Isolation and characterization of Df(2L)BSC521 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC521 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08987 and PBac{WH}CG31665[f00122]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}d08987/PBac{WH}CG31665[f00122] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC521 from the segment of P{XP}d08987 to the left of its FRT site and the segment of PBac{WH}CG31665[f00122] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC521 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 22A5;22B3. Df(2L)BSC521 failed to complement cpb[M143]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX