From: Kevin Cook <kcook@XXXX> To: flybase-updates@XXXX Cc: Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(3R)BSC423 Date: Tue, 15 Apr 2008 14:24:59 -0400 ( 19:24 BST) Isolation and characterization of Df(3R)BSC423 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC423 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09643 and PBac{WH}f05976. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}d09643/PBac{WH}f05976 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC423 from the segment of P{XP}d09643 to the left of its FRT site and the segment of PBac{WH}f05976 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC423 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 84D1;84D5. Df(3R)BSC423 failed to complement lap[1], Gld[n2] and rn[16]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX