From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC420
Date: 	Tue, 15 Apr 2008  14:22:41  -0400  ( 19:22  BST)
Isolation and characterization of Df(3L)BSC420
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC420 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}RpLP0[d09567] and PBac{WH}f01548. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}RpLP0[d09567]/PBac{WH}f01548 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC420 from the segment of 
P{XP}RpLP0[d09567] to the left of its FRT site and the segment of 
PBac{WH}f01548 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC420 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 79B2;79F1. Df(3L)BSC420 failed to complement Hem[03335], 
Aats-ile[00827] and Ten-m[05309].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX