From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(2L)BSC480
Date: 	Tue, 15 Apr 2008  15:01:03  -0400  ( 20:01  BST)
Isolation and characterization of Df(2L)BSC480
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC480 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d08987 and PBac{WH}f07098. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d08987/PBac{WH}f07098 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC480 from the segment of P{XP}d08987 
to the left of its FRT site and the segment of PBac{WH}f07098 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(2L)BSC480 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
22A5;22C3. Df(2L)BSC480 failed to complement cpb[M143] and cpb[F19].