From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC446
Date: 	Tue, 15 Apr 2008  14:39:40  -0400  ( 19:39  BST)
Isolation and characterization of Df(3L)BSC446
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC446 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f00301 and P{XP}d09071. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f00301/P{XP}d09071 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC446 from the segment of 
PBac{WH}f00301 to the left of its FRT site and the segment of 
P{XP}d09071 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(3L)BSC446 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
76F1;77A1. Df(3L)BSC446 failed to complement gig[109] and Df(3L)XS533.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX