To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC375
Date: Mon, 29 Oct 2007  12:07:20  -0400
Isolation and characterization of Df(3L)BSC375
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC375 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f03786 and P{XP}pbl[d06970]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f03786/P{XP}pbl[d06970] males. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC375 from the segment of 
PBac{WH}f03786 to the left of its FRT site and the segment of 
P{XP}pbl[d06970] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC375 predicted from the 
Release 5 genomic coordinates of the transposable element insertion 
sites are 66A3;66A19. Df(3L)BSC375 failed to complement Pdp1[P205] 
and pbl[3]. Df(3L)FDD-0325408 is a synonym for Df(3L)BSC375.