To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC381
Date: Mon, 29 Oct 2007  12:10:41  -0400
Isolation and characterization of Df(3L)BSC381
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC381 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG10638[f01666] and P{XP}CG14118[d06432]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; PBac{WH}CG10638[f01666]/P{XP}CG14118[d06432] males. 
These males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC381 from the segment of PBac{WH}CG10638[f01666]  to 
the left of its FRT site and the segment of P{XP}CG14118[d06432] to 
the right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC381 predicted from the Release 5 genomic 
coordinates of the transposable element insertion sites are 
69C4;69E8. Df(3L)BSC381 failed to complement Ptp69D[1].