Date: Mon, 29 Oct 2007  12:07:21  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC396
Isolation and characterization of Df(3L)BSC396
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC396 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d09934 and PBac{RB}CG5645[e03479]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d09934/PBac{RB}CG5645[e03479] males. These males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.RB3}BSC396 from the segment of 
P{XP}d09934 to the left of its FRT site and the segment of 
PBac{RB}CG5645[e03479] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(3L)BSC396 predicted from the Release 5 genomic coordinates of the 
transposable element insertion sites are 68F3;68F5. Df(3L)BSC396 
failed to complement l(3)j2D3[j2D3]. Df(3L)FDD-0110089 is a synonym 
for Df(3L)BSC396.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX