Date: Mon, 29 Oct 2007  12:11:55  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC388
Isolation and characterization of Df(3L)BSC388
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC388 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d03852 and PBac{RB}e03262. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; 
P{XP}d03852/PBac{RB}e03262 males. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC388 from the segment of P{XP}d03852 
to the left of its FRT site and the segment of PBac{RB}e03262 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. with the substitution 
of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or 
RB3' minus primer in the Hybrid PCR protocol in the Supplementary 
Methods. The cytological breakpoints of Df(3L)BSC388 predicted from 
the Release 5 genomic coordinates of the transposable element 
insertion sites are 66A8;66B11. Df(3L)BSC388 failed to complement 
Pdp1[P205] and pbl[3].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX