To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC369
Date: Mon, 29 Oct 2007  12:04:54  -0400
Isolation and characterization of Df(3L)BSC369
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC369 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f04973a and P{XP}CG14998[d02685]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; PBac{WH}f04973a/P{XP}CG14998[d02685] males. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC369 from the segment 
of PBac{WH}f04973a to the left of its FRT site and the segment of 
P{XP}CG14998[d02685] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC369 predicted from the 
Release 5 genomic coordinates of the transposable element insertion 
sites are 64A1;64A7. Df(3L)BSC369 failed to complement wit[A12] and Faa[A9].