From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(1)BSC405
Date: 	Mon, 29 Oct 2007  12:10:41  -0400  ( 16:10  GMT)
Isolation and characterization of Df(1)BSC405
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC405 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d03790 and PBac{RB}e00459. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d03790/PBac{RB}e00459 MKRS, P{hsFLP}86E/+ females crossed to 
Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC405 from the segment of P{XP}d03790 
to the left of its FRT site and the segment of PBac{RB}e00459 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. with the substitution 
of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or 
RB3' minus primer in the Hybrid PCR protocol in the Supplementary 
Methods. The cytological breakpoints of Df(1)BSC405 predicted from 
the Release 5 genomic coordinates of the transposable element 
insertions sites are 16D5;16F6. The presence of a deletion was 
confirmed cytologically, though the breakpoints were not analyzed in detail.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX