From: Kevin Cook <kcook@XXXX> To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(1)BSC315 Date: Mon, 29 Oct 2007 12:07:15 -0400 ( 16:07 GMT) Isolation and characterization of Df(1)BSC315 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC315 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03544 and PBac{WH}CG7536[f03720]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}d03544/PBac{WH}CG7536[f03720]; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC315 from the segment of P{XP}d03544 to the left of its FRT site and the segment of PBac{WH}CG7536[f03720]to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC315 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 16B1;16F6. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
