Date: Wed, 30 Aug 2006  15:14:24  -0400
From: Kevin Cook <kcook@XXXX>
To: flybase-updates@XXXX
CC: kaufmanXXXX, Stacey Christensen <sjchristXXXX>, 
        Jill Gresens <jgresensXXXX>, mdealXXXX
Isolation and characterization of Df(2L)BSC206
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC206 was isolated as a FLP recombinase-induced recombination
event involving PBac{RB}me31B[e03055] and P{XP}d05337a. The deletion
was isolated as a chromosome lacking miniwhite markers in progeny of
P{hsFLP}1, y[1] w[1118]; PBac{RB}me31B[e03055]/P{XP}d05337a males
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males
were heat shocked as larvae as described in Parks et al., Nature
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in
preceding and succeeding generations maintained the original genetic
background of the Exelixis insertion stocks (Thibault et al., Nature
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event
generated the genetic element P+PBac{XP5.RB3}BSC206 from the segment
of PBac{RB}me31B[e03055] to the left of its FRT site and the segment
of P{XP}d05337a to the right of its FRT site. Its presence was
verified using the PCR methods and primers described in Parks et al
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3'
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in
the Supplementary Methods. The cytological breakpoints of
Df(2L)BSC206 predicted from the transposable element insertions sites
using Release 4 coordinates are 31B1;31D9. It failed to complement
chico[1], trk[1] and bsk[1] .
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX