[31B1-31B1];[31D9-31D9];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
31B1;31D9
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Presence of P+PBac{XP5.RB3}BSC206 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3'for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(2L)BSC206 predicted from the PBac{RB}me31Be03055 and P{XP}d05337a transposable element insertions sites
using Release 4 coordinates are 31B1;31D9.