Date: Tue, 08 May 2007 08:46:15 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC302 Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX Isolation and characterization of Df(2L)BSC302 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC302 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG9265[d00690] and PBac{WH}CG9249[f00835]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}CG9265[d00690]/PBac{WH}CG9249[f00835] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC302 from the segment of P{XP}CG9265[d00690] to the left of its FRT site and the segment of PBac{WH}CG9249[f00835] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC302 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 39A1;39A6. It failed to complement Mpps[k16403] and Df(2L)Exel7080. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX