Date: Tue, 29 Aug 2006  15:38:03  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC172
Cc: kaufmanXXXX, mdealXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX>
Isolation and characterization of Df(2L)BSC172
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC172 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d01421 and PBac{RB}e03037. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d01421/PBac{RB}e03037 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC172 from the segment of P{XP}d01421 
to the left of its FRT site and the segment of PBac{RB}e03037 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al with the substitution of 
the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' 
minus primer in the Hybrid PCR protocol in the Supplementary Methods. 
The cytological breakpoints of Df(2L)BSC172 predicted from the 
Release 4 genomic coordinates of the transposable element insertion 
sites are 25B10;25C1. It failed to complement vkg[01209].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX