Date: Tue, 29 Aug 2006 15:38:08 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC167 Cc: kaufmanXXXX, mdealXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX> Isolation and characterization of Df(2L)BSC167 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC167 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG31989[f02191] and P{XP}d04336. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{WH}CG31989[f02191]/P{XP}d04336 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC167 from the segment of PBac{WH}CG31989[f02191] to the left of its FRT site and the segment of P{XP}d04336 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC167 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 25E5;25E6. It failed to complement CG7277[KG03584]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
