Date: Tue, 08 May 2007  08:46:12  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC289
Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Isolation and characterization of Df(3L)BSC289
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC289 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG2213[f05423] and P{XP}d04894. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}CG2213[f05423]/P{XP}d04894 males. The males were 
heat shocked as larvae as described in Parks et al. Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC289 from the segment of 
PBac{WH}CG2213[f05423] to the left of its FRT site and the segment of 
P{XP}d04894 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(3L)BSC289 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
61F6;62A9. Df(3L)BSC289 failed to complement Df(3L)ED207, 
Df(3L)ED4256, cue[2] and pUf68[EY07952].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX