Date: Tue, 08 May 2007  08:45:54  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC278
Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Isolation and characterization of Df(2L)BSC278
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC278 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d07167 and PBac{RB}pkaap[e04176]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d07167/PBac{RB}pkaap[e04176] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.RB3}BSC278 from the segment 
of P{XP}d07167 to the left of its FRT site and the segment of 
PBac{RB}pkaap[e04176] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(2L)BSC278 predicted from the Release 5 genomic coordinates of the 
transposable element insertions sites are 35E1;35F1. It failed to 
complement BicC[4] and crp[k00809].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX