Date: Thu, 22 Mar 2007 14:52:07 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC253 Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufman@XXXX Isolation and characterization of Df(2L)BSC253 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC253 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03477 and PBac{WH}nAcRalpha-34E[f00872]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}d03477/PBac{WH}nAcRalpha-34E[f00872] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC253 from the segment of P{XP}d03477 to the left of its FRT site and the segment of PBac{WH}nAcRalpha-34E[f00872] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d03477 to be at Release 3 genomic coordinate 13860811 on chromosome arm 2L. This corresponds to 34E1 on the Release 3 and 4 genome maps. The predicted position of PBac{WH}nAcRalpha-34E[f00872] on the Release 4 map is 34F3. Consequently, the cytological breakpoints of Df(2L)BSC253 are predicted to be 34E1;34F3. It failed to complement Ance[34Eb-2] and rk[4]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
