Date: Tue, 29 Aug 2006  15:38:01  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC174
Isolation and characterization of Df(3R)BSC174
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC174 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f00382 and P{XP}CG31531[d00921]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f00382/P{XP}CG31531[d00921] males. The males were 
heat shocked as larvae as described in Parks et al. Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC174 from the segment of 
PBac{WH}f00382 to the left of its FRT site and the segment of 
P{XP}CG31531[d00921] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC174 predicted from the 
transposable element insertions sites using Release 4 coordinates are 
82C1;82D1. It failed to complement Karybeta3[j3A4].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX