Date: Tue, 05 Dec 2006  14:53:57  -0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC221
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(3R)BSC221
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC221 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d11515 and PBac{WH}f00935. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d11515/PBac{WH}f00935 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC221 from the segment of P{XP}d11515 
to the left of its FRT site and the segment of PBac{WH}f00935 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. Exelixis, Inc. 
determined the insertion site of P{XP}d11515 to be at Release 3 
genomic coordinate 2933219 on chromosome arm 3R. The Gene Disruption 
Project determined the insertion site of P{XP}d11515 to be at Release 
3 genomic coordinate 2933501 on arm 3R. This corresponds to 84C1 on 
both the Release 3 and Release 4 genome maps. The predicted position 
of PBac{WH}f00935 on the Release 4 map is 84D2. Consequently, the 
cytological breakpoints of Df(3R)BSC221 are predicted to be 
84C1;84D2. Df(3R)BSC221 failed to complement lap[1], sas[15] and 
l(3)s2214[s2214].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX