To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC127
Cc: Stacey Christensen <sjchrist_at_indiana.edu>, mdeal_at_indiana.edu, Jill
Gresens <jgresens_at_indiana.edu>, Rachel Andrade
<r-andrade_at_md.northwestern.edu>, kaufman_at_bio.indiana.edu
Isolation and characterization of Df(3L)BSC127
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC127 was isolated as a FLP recombinase-induced recombination
event involving PBac{WH}drprf04411 and P{XP}d06594. The deletion
was isolated as a chromosome lacking miniwhite markers in progeny of
w1118, P{hsFLP}1; PBac{WH}drprf04411/P{XP}d06594 females crossed
to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females
were heat shocked as larvae as described in Parks et al., Nature
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in
preceding and succeeding generations maintained the original genetic
background of the Exelixis insertion stocks (Thibault et al., Nature
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event
generated the genetic element P+PBac{XP5.WH5}BSC127 from the segment
of PBac{WH}drprf04411 to the left of its FRT site and the segment
of P{XP}d06594 to the right of its FRT site. Its presence was
verified using the PCR methods and primers described in Parks et
al. Exelixis, Inc. determined the insertion site of P{XP}d06594 to
be at Release 3 genomic coordinate 1774835 on arm 3L. The Gene
Disruption project determined the insertion site of P{XP}d06594 to
be at Release 3 genomic coordinate 1774992 on arm 3L. The cytological
breakpoints of Df(3L)BSC127 predicted from the transposable element
insertions sites are 62B1;62B4. It failed to complement dlt04276,
Df(3L)Aprt-1 and Df(3L)Exel6088.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX